Ontology highlight
ABSTRACT:
SUBMITTER: Eriani G
PROVIDER: S-EPMC333589 | biostudies-other | 1991 Jan
REPOSITORIES: biostudies-other
Eriani G G Dirheimer G G Gangloff J J
Nucleic acids research 19910101 2
The gene coding for E. coli cysteinyl-tRNA synthetase (cysS) was isolated by complementation of a strain deficient in cysteinyl-tRNA synthetase activity at high temperature (43 degrees C). Sequencing of a 2.1 kbp DNA fragment revealed an open reading frame of 1383 bp coding for a protein of 461 amino acid residues with a Mr of 52,280, a value in close agreement with that observed for the purified protein, which behaves as a monomer. The sequence of CysRS bears the canonical His-Ile- Gly -His (HI ...[more]