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Nucleotide sequence of Hungarian grapevine chrome mosaic nepovirus RNA1.


ABSTRACT: The nucleotide sequence of the RNA1 of hungarian grapevine chrome mosaic virus, a nepovirus very closely related to tomato black ring virus, has been determined from cDNA clones. It is 7212 nucleotides in length excluding the 3' terminal poly(A) tail and contains a large open reading frame extending from nucleotides 216 to 6971. The presumably encoded polyprotein is 2252 amino acids in length with a molecular weight of 250 kDa. The primary structure of the polyprotein was compared with that of other viral polyproteins, revealing the same general genetic organization as that of other picorna-like viruses (comoviruses, potyviruses and picornaviruses), except that an additional protein is suspected to occupy the N-terminus of the polyprotein.

SUBMITTER: Le Gall O 

PROVIDER: S-EPMC334887 | biostudies-other | 1989 Oct

REPOSITORIES: biostudies-other

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Nucleotide sequence of Hungarian grapevine chrome mosaic nepovirus RNA1.

Le Gall O O   Candresse T T   Brault V V   Dunez J J  

Nucleic acids research 19891001 19


The nucleotide sequence of the RNA1 of hungarian grapevine chrome mosaic virus, a nepovirus very closely related to tomato black ring virus, has been determined from cDNA clones. It is 7212 nucleotides in length excluding the 3' terminal poly(A) tail and contains a large open reading frame extending from nucleotides 216 to 6971. The presumably encoded polyprotein is 2252 amino acids in length with a molecular weight of 250 kDa. The primary structure of the polyprotein was compared with that of o  ...[more]

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