A DNA polymerase from the archaeon Sulfolobus solfataricus shows sequence similarity to family B DNA polymerases.
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ABSTRACT:
SUBMITTER: Pisani FM
PROVIDER: S-EPMC336912 | biostudies-other | 1992 Jun
REPOSITORIES: biostudies-other
Publications
A DNA polymerase from the archaeon Sulfolobus solfataricus shows sequence similarity to family B DNA polymerases.
Pisani F M FM De Martino C C Rossi M M
Nucleic acids research 19920601 11
The gene encoding the thermostable DNA polymerase from the archaeon Sulfolobus solfataricus (strain MT 4) was isolated by means of two degenerate oligonucleotide probes. They were designed on the basis of partial enzyme amino acid sequences. The gene was found to encode a 882 residues polypeptide chain with a deduced molecular mass of about 100 kDa. By comparison with other archaeal genes, putative regulatory sites were identified in the gene-flanking regions. By computer-assisted homology searc ...[more]
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