Heterologous activation of the actinorhodin biosynthetic pathway in Streptomyces lividans.
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ABSTRACT:
SUBMITTER: Romero NM
PROVIDER: S-EPMC336920 | biostudies-other | 1992 Jun
REPOSITORIES: biostudies-other
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Heterologous activation of the actinorhodin biosynthetic pathway in Streptomyces lividans.
Romero N M NM Parro V V Malpartida F F Mellado R P RP
Nucleic acids research 19920601 11
A DNA fragment of Streptomyces fradiae is able to activate the antibiotic actinorhodin biosynthetic pathway when cloned in Streptomyces lividans. The activator DNA region has been sequenced and its transcription initiation and termination sites accurately mapped in vivo. This DNA encodes a 132 nucleotides long transcript which is apparently responsible for the actinorhodin production phenotype, possibly acting as an antisense RNA. The sequence of the activator gene revealed no homology with any ...[more]
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