Unknown

Dataset Information

0

Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta.


ABSTRACT: The tumor suppressor p53, a sensor of multiple forms of cellular stress, is regulated by post-translational mechanisms to induce cell-cycle arrest, senescence, or apoptosis. We demonstrate that endoplasmic reticulum (ER) stress inhibits p53-mediated apoptosis. The mechanism of inhibition involves the increased cytoplasmic localization of p53 due to phosphorylation at serine 315 and serine 376, which is mediated by glycogen synthase kinase-3 beta (GSK-3beta). ER stress induces GSK-3beta binding to p53 in the nucleus and enhances the cytoplasmic localization of the tumor suppressor. Inhibition of apoptosis caused by ER stress requires GSK-3beta and does not occur in cells expressing p53 with mutation(s) of serine 315 and/or serine 376 to alanine(s). As a result of the increased cytoplasmic localization, ER stress prevents p53 stabilization and p53-mediated apoptosis upon DNA damage. It is concluded that inactivation of p53 is a protective mechanism utilized by cells to adapt to ER stress.

SUBMITTER: Qu L 

PROVIDER: S-EPMC338280 | biostudies-other | 2004 Feb

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC1782373 | biostudies-literature
| S-EPMC3429326 | biostudies-literature
| S-EPMC4330769 | biostudies-literature
| S-EPMC11648764 | biostudies-literature
| S-EPMC5876643 | biostudies-literature
| S-EPMC7189383 | biostudies-literature
| S-EPMC10579530 | biostudies-literature
| S-EPMC7838309 | biostudies-literature
| S-EPMC3698985 | biostudies-literature
| S-EPMC8875608 | biostudies-literature