Ontology highlight
ABSTRACT:
SUBMITTER: Lian X
PROVIDER: S-EPMC3390875 | biostudies-other | 2012 Jul
REPOSITORIES: biostudies-other
Lian Xiaojun X Hsiao Cheston C Wilson Gisela G Zhu Kexian K Hazeltine Laurie B LB Azarin Samira M SM Raval Kunil K KK Zhang Jianhua J Kamp Timothy J TJ Palecek Sean P SP
Proceedings of the National Academy of Sciences of the United States of America 20120529 27
Human pluripotent stem cells (hPSCs) offer the potential to generate large numbers of functional cardiomyocytes from clonal and patient-specific cell sources. Here we show that temporal modulation of Wnt signaling is both essential and sufficient for efficient cardiac induction in hPSCs under defined, growth factor-free conditions. shRNA knockdown of β-catenin during the initial stage of hPSC differentiation fully blocked cardiomyocyte specification, whereas glycogen synthase kinase 3 inhibition ...[more]