Ontology highlight
ABSTRACT:
SUBMITTER: Chatterjee N
PROVIDER: S-EPMC3422235 | biostudies-other | 2012
REPOSITORIES: biostudies-other
Chatterjee Nilanjana N Banerjee Tanmay T Datta Santanu S
PloS one 20120817 8
Accurate estimation of template--DNA or RNA by real time PCR is dependent on the amplification efficiency (F) of the reaction. The analytical equation describing the kinetics of PCR that is influenced by template re-annealing is formulated. It predicts the gradual reduction of F--from its initial value of 2, leading to template saturation. From an experimental standpoint, due to the exponential nature of the reaction a minute change in F can lead to a large error in the estimation of the initial ...[more]