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Optical stretching of giant unilamellar vesicles with an integrated dual-beam optical trap.


ABSTRACT: We have integrated a dual-beam optical trap into a microfluidic platform and used it to study membrane mechanics in giant unilamellar vesicles (GUVs). We demonstrate the trapping and stretching of GUVs and characterize the membrane response to a step stress. We then measure area strain as a function of applied stress to extract the bending modulus of the lipid bilayer in the low-tension regime.

SUBMITTER: Solmaz ME 

PROVIDER: S-EPMC3469987 | biostudies-other | 2012 Oct

REPOSITORIES: biostudies-other

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