Ontology highlight
ABSTRACT:
SUBMITTER: Guo M
PROVIDER: S-EPMC3497798 | biostudies-other | 2012 Oct
REPOSITORIES: biostudies-other
Guo Minghao M Xu Yangfan Y Gruebele Martin M
Proceedings of the National Academy of Sciences of the United States of America 20120604 44
We measure the stability and folding rate of a mutant of the enzyme phosphoglycerate kinase (PGK) inside bone tissue cells as a function of temperature from 38 to 48 °C. To facilitate measurement in individual living cells, we developed a rapid laser temperature stepping method capable of measuring complete thermal melts and kinetic traces in about two min. We find that this method yields improved thermal melts compared to heating a sample chamber or microscope stage. By comparing results for si ...[more]