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Preparation of coaxial-electrospun poly[bis(p-methylphenoxy)]phosphazene nanofiber membrane for enzyme immobilization.

ABSTRACT: A core/sheath nanofiber membrane with poly[bis(p-methylphenoxy)]phospha-zene (PMPPh) as the sheath and easily spinnable polyacrylonitrile (PAN) as the core was prepared via a coaxial electrospinning process. Field-emission scanning electron microscopy and transmission electron microscopy were used to characterize the morphology of the nanofiber membrane. It was found that the concentration of the PAN spinning solution and the ratio of the core/sheath solution flow rates played a decisive role in the coaxial electrospinning process. In addition, the stabilized core/sheath PMPPh nanofiber membrane was investigated as a support for enzyme immobilization because of its excellent biocompatibility, high surface/volume ratio, and large porosity. Lipase from Candida rugosa was immobilized on the nanofiber membrane by adsorption. The properties of the immobilized lipase on the polyphosphazene nanofiber membrane were studied and compared with those of a PAN nanofiber membrane. The results showed that the adsorption capacity (20.4 ± 2.7 mg/g) and activity retention (63.7%) of the immobilized lipase on the polyphosphazene nanofiber membrane were higher than those on the PAN membrane.

SUBMITTER: Wang SG

PROVIDER: S-EPMC3509571 | biostudies-other | 2012

REPOSITORIES: biostudies-other

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Preparation of coaxial-electrospun poly[bis(p-methylphenoxy)]phosphazene nanofiber membrane for enzyme immobilization.

Wang Shu-Gen SG Jiang Xin X Chen Peng-Cheng PC Yu An-Guo AG Huang Xiao-Jun XJ

International journal of molecular sciences 20121102 11

A core/sheath nanofiber membrane with poly[bis(p-methylphenoxy)]phospha-zene (PMPPh) as the sheath and easily spinnable polyacrylonitrile (PAN) as the core was prepared via a coaxial electrospinning process. Field-emission scanning electron microscopy and transmission electron microscopy were used to characterize the morphology of the nanofiber membrane. It was found that the concentration of the PAN spinning solution and the ratio of the core/sheath solution flow rates played a decisive role in ...[more]

PMID: 23203055

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Dataset Information

Preparation of coaxial-electrospun poly[bis(p-methylphenoxy)]phosphazene nanofiber membrane for enzyme immobilization.

Publications

Preparation of coaxial-electrospun poly[bis(p-methylphenoxy)]phosphazene nanofiber membrane for enzyme immobilization.

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