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Immunoproteomic analysis of potential serum biomarker candidates in human glaucoma.

ABSTRACT: Evidence supporting the immune system involvement in glaucoma includes increased titers of serum antibodies to retina and optic nerve proteins, although their pathogenic importance remains unclear. This study using an antibody-based proteomics approach aimed to identify disease-related antigens as candidate biomarkers of glaucoma.Serum samples were collected from 111 patients with primary open-angle glaucoma and an age-matched control group of 49 healthy subjects without glaucoma. For high-throughput characterization of antigens, serum IgG was eluted from five randomly selected glaucomatous samples and analyzed by linear ion trap mass spectrometry (LC-MS/MS). Serum titers of selected biomarker candidates were then measured by specific ELISAs in the whole sample pool (including an additional control group of diabetic retinopathy).LC-MS/MS analysis of IgG elutes revealed a complex panel of proteins, including those detectable only in glaucomatous samples. Interestingly, many of these antigens corresponded to upregulated retinal proteins previously identified in glaucomatous donors (or that exhibited increased methionine oxidation). Moreover, additional analysis detected a greater immunoreactivity of the patient sera to glaucomatous retinal proteins (or to oxidatively stressed cell culture proteins), thereby suggesting the importance of disease-related protein modifications in autoantibody production/reactivity. As a narrowing-down strategy for selection of initial biomarker candidates, we determined the serum proteins overlapping with the retinal proteins known to be up-regulated in glaucoma. Four of the selected 10 candidates (AIF, cyclic AMP-responsive element binding protein, ephrin type-A receptor, and huntingtin) exhibited higher ELISA titers in the glaucomatous sera.A number of serum proteins identified by this immunoproteomic study of human glaucoma may represent diseased tissue-related antigens and serve as candidate biomarkers of glaucoma.

SUBMITTER: Tezel G

PROVIDER: S-EPMC3522442 | biostudies-other | 2012 Dec

REPOSITORIES: biostudies-other

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Immunoproteomic analysis of potential serum biomarker candidates in human glaucoma.

Tezel Gülgün G Thornton Ivey L IL Tong Melissa G MG Luo Cheng C Yang Xiangjun X Cai Jian J Powell David W DW Soltau Joern B JB Liebmann Jeffrey M JM Ritch Robert R

Investigative ophthalmology & visual science 20121213 13

<h4>Purpose</h4>Evidence supporting the immune system involvement in glaucoma includes increased titers of serum antibodies to retina and optic nerve proteins, although their pathogenic importance remains unclear. This study using an antibody-based proteomics approach aimed to identify disease-related antigens as candidate biomarkers of glaucoma.<h4>Methods</h4>Serum samples were collected from 111 patients with primary open-angle glaucoma and an age-matched control group of 49 healthy subjects ...[more]

PMID: 23150628

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Immunoproteomic analysis of potential serum biomarker candidates in human glaucoma.

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Immunoproteomic analysis of potential serum biomarker candidates in human glaucoma.

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