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Clinical impact of circulating miR-221 in plasma of patients with pancreatic cancer.

ABSTRACT: BACKGROUND: Several recent studies have demonstrated that microRNAs (miRNAs) are stably detectable in plasma/serum. We tested miR-221 and miR-375, which are frequently reported to be highly and poorly expressed in pancreatic cancer (PCa), as candidates for plasma biomarkers in PCa. METHODS: This study was divided into three parts: (1) Confirmation of higher miR-221 levels in primary PCa tissue and cell lines than normal pancreatic tissues. (2) Evaluation of plasma miR-221 and miR-375 concentrations by comparing results from 47 consecutive PCa patients and 30 healthy volunteers. (3) Evaluation of the assay for monitoring tumour dynamics in PCa patients. RESULTS: (1) Expression of miR-221 was significantly higher in PCa tissues and cell lines than normal pancreatic tissues. (2) Plasma miR-221 concentrations were significantly higher in PCa patients than that in benign pancreatic tumours (P=0.016) and controls (P<0.0005), while plasma miR-375 concentrations tended to be lower in PCa patients (P=0.064), and the miR-221/miR-375 ratio was significantly higher (P<0.0001) in PCa patients than in controls. (3) Plasma miR-221 concentrations were significantly reduced in postoperative samples (P=0.018). Furthermore, PCa patients with high plasma miR-221 concentrations had significant correlation with distant metastasis (P=0.041), and non-resectable status (P=0.021). CONCLUSION: Plasma miR-221 could be a useful biomarker for cancer detection, monitoring tumour dynamics and predicting malignant outcomes in PCa patients, and may contribute to clinical decision making in PCa treatments.

SUBMITTER: Kawaguchi T

PROVIDER: S-EPMC3566805 | biostudies-other | 2013 Feb

REPOSITORIES: biostudies-other

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Clinical impact of circulating miR-221 in plasma of patients with pancreatic cancer.

Kawaguchi T T Komatsu S S Ichikawa D D Morimura R R Tsujiura M M Konishi H H Takeshita H H Nagata H H Arita T T Hirajima S S Shiozaki A A Ikoma H H Okamoto K K Ochiai T T Taniguchi H H Otsuji E E

British journal of cancer 20130117 2

<h4>Background</h4>Several recent studies have demonstrated that microRNAs (miRNAs) are stably detectable in plasma/serum. We tested miR-221 and miR-375, which are frequently reported to be highly and poorly expressed in pancreatic cancer (PCa), as candidates for plasma biomarkers in PCa.<h4>Methods</h4>This study was divided into three parts: (1) Confirmation of higher miR-221 levels in primary PCa tissue and cell lines than normal pancreatic tissues. (2) Evaluation of plasma miR-221 and miR-37 ...[more]

PMID: 23329235

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Dataset Information

Clinical impact of circulating miR-221 in plasma of patients with pancreatic cancer.

Publications

Clinical impact of circulating miR-221 in plasma of patients with pancreatic cancer.

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