Ontology highlight
ABSTRACT:
SUBMITTER: Chen S
PROVIDER: S-EPMC3575824 | biostudies-other | 2013 Feb
REPOSITORIES: biostudies-other
Chen Shijia S Oikonomou Grigorios G Chiu Cindy N CN Niles Brett J BJ Liu Justin J Lee Daniel A DA Antoshechkin Igor I Prober David A DA
Nucleic acids research 20130108 4
Zinc-finger nucleases (ZFNs) and TAL effector nucleases (TALENs) have been shown to induce targeted mutations, but they have not been extensively tested in any animal model. Here, we describe a large-scale comparison of ZFN and TALEN mutagenicity in zebrafish. Using deep sequencing, we found that TALENs are significantly more likely to be mutagenic and induce an average of 10-fold more mutations than ZFNs. We observed a strong correlation between somatic and germ-line mutagenicity, and identifie ...[more]