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Susceptibility of L-FABP-/- mice to oxidative stress in early-stage alcoholic liver.

ABSTRACT: Chronic ethanol consumption is a prominent cause of liver disease worldwide. Dysregulation of an important lipid uptake and trafficking gene, liver-fatty acid binding protein (L-FABP), may contribute to alterations in lipid homeostasis during early-stage alcoholic liver. We have reported the detrimental effects of ethanol on the expression of L-FABP and hypothesize this may deleteriously impact metabolic networks regulating fatty acids. Male wild-type (WT) and L-FABP(-/-) mice were fed a modified Lieber-DeCarli liquid diet for six weeks. To assess the response to chronic ethanol ingestion, standard biochemical indicators for alcoholic liver disease (ALD) and oxidative stress were measured. Ethanol ingestion resulted in attenuation of hepatic triglyceride accumulation and elevation of cholesterol in L-FABP(-/-) mice. Lipidomics analysis validated multiple alterations in hepatic lipids resulting from ethanol treatment. Increased immunohistochemical staining for the reactive aldehydes 4-hydroxynonenal and malondialdehyde were observed in WT mice ingesting ethanol; however, L-FABP(-/-) mice displayed prominent protein adducts in liver sections evaluated from pair-fed and ethanol-fed mice. Likewise, alterations in glutathione, thiobarbituric acid reactive substances (TBARS), 8-isoprostanes, and protein carbonyl content all indicated L-FABP(-/-) mice exhibit high sustained oxidative stress in the liver. These data establish that L-FABP is an indirect antioxidant protein essential for sequestering FFA and that its impairment could contribute to in the pathogenesis of ALD.

SUBMITTER: Smathers RL

PROVIDER: S-EPMC3622328 | biostudies-other | 2013 May

REPOSITORIES: biostudies-other

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Susceptibility of L-FABP-/- mice to oxidative stress in early-stage alcoholic liver.

Smathers Rebecca L RL Galligan James J JJ Shearn Colin T CT Fritz Kristofer S KS Mercer Kelly K Ronis Martin M Orlicky David J DJ Davidson Nicholas O NO Petersen Dennis R DR

Journal of lipid research 20130128 5

Chronic ethanol consumption is a prominent cause of liver disease worldwide. Dysregulation of an important lipid uptake and trafficking gene, liver-fatty acid binding protein (L-FABP), may contribute to alterations in lipid homeostasis during early-stage alcoholic liver. We have reported the detrimental effects of ethanol on the expression of L-FABP and hypothesize this may deleteriously impact metabolic networks regulating fatty acids. Male wild-type (WT) and L-FABP(-/-) mice were fed a modifie ...[more]

PMID: 23359610

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Susceptibility of L-FABP-/- mice to oxidative stress in early-stage alcoholic liver.

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Susceptibility of L-FABP-/- mice to oxidative stress in early-stage alcoholic liver.

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