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On-chip cytometry using plasmonic nanoparticle enhanced lensfree holography.

ABSTRACT: Computational microscopy tools, in particular lensfree on-chip imaging, provide a large field-of-view along with a long depth-of-field, which makes it feasible to rapidly analyze large volumes of specimen using a compact and light-weight on-chip imaging architecture. To bring molecular specificity to this high-throughput platform, here we demonstrate the use of plasmon-resonant metallic nanoparticles to automatically recognize different cell types based on their plasmon-enhanced lensfree holograms, detected and reconstructed over a large field-of-view of e.g., ~24?mm².

SUBMITTER: Wei Q

PROVIDER: S-EPMC3632884 | biostudies-other | 2013

REPOSITORIES: biostudies-other

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On-chip cytometry using plasmonic nanoparticle enhanced lensfree holography.

Wei Qingshan Q McLeod Euan E Qi Hangfei H Wan Zhe Z Sun Ren R Ozcan Aydogan A

Scientific reports 20130101

Computational microscopy tools, in particular lensfree on-chip imaging, provide a large field-of-view along with a long depth-of-field, which makes it feasible to rapidly analyze large volumes of specimen using a compact and light-weight on-chip imaging architecture. To bring molecular specificity to this high-throughput platform, here we demonstrate the use of plasmon-resonant metallic nanoparticles to automatically recognize different cell types based on their plasmon-enhanced lensfree hologra ...[more]

PMID: 23608952

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