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Hydrogen sulfide induces systemic tolerance to salinity and non-ionic osmotic stress in strawberry plants through modification of reactive species biosynthesis and transcriptional regulation of multiple defence pathways.

ABSTRACT: Hydrogen sulfide (H2S) has been recently found to act as a potent priming agent. This study explored the hypothesis that hydroponic pretreatment of strawberry (Fragaria × ananassa cv. Camarosa) roots with a H2S donor, sodium hydrosulfide (NaHS; 100 ?M for 48 h), could induce long-lasting priming effects and tolerance to subsequent exposure to 100mM NaCI or 10% (w/v) PEG-6000 for 7 d. Hydrogen sulfide pretreatment of roots resulted in increased leaf chlorophyll fluorescence, stomatal conductance and leaf relative water content as well as lower lipid peroxidation levels in comparison with plants directly subjected to salt and non-ionic osmotic stress, thus suggesting a systemic mitigating effect of H2S pretreatment to cellular damage derived from abiotic stress factors. In addition, root pretreatment with NaHS resulted in the minimization of oxidative and nitrosative stress in strawberry plants, manifested via lower levels of synthesis of NO and H(2)O(2) in leaves and the maintenance of high ascorbate and glutathione redox states, following subsequent salt and non-ionic osmotic stresses. Quantitative real-time RT-PCR gene expression analysis of key antioxidant (cAPX, CAT, MnSOD, GR), ascorbate and glutathione biosynthesis (GCS, GDH, GS), transcription factor (DREB), and salt overly sensitive (SOS) pathway (SOS2-like, SOS3-like, SOS4) genes suggests that H2S plays a pivotal role in the coordinated regulation of multiple transcriptional pathways. The ameliorative effects of H2S were more pronounced in strawberry plants subjected to both stress conditions immediately after NaHS root pretreatment, rather than in plants subjected to stress conditions 3 d after root pretreatment. Overall, H2S-pretreated plants managed to overcome the deleterious effects of salt and non-ionic osmotic stress by controlling oxidative and nitrosative cellular damage through increased performance of antioxidant mechanisms and the coordinated regulation of the SOS pathway, thus proposing a novel role for H2S in plant priming, and in particular in a fruit crop such as strawberry.

SUBMITTER: Christou A

PROVIDER: S-EPMC3638822 | biostudies-other | 2013 Apr

REPOSITORIES: biostudies-other

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Hydrogen sulfide induces systemic tolerance to salinity and non-ionic osmotic stress in strawberry plants through modification of reactive species biosynthesis and transcriptional regulation of multiple defence pathways.

Christou Anastasis A Manganaris George A GA Papadopoulos Ioannis I Fotopoulos Vasileios V

Journal of experimental botany 20130408 7

Hydrogen sulfide (H2S) has been recently found to act as a potent priming agent. This study explored the hypothesis that hydroponic pretreatment of strawberry (Fragaria × ananassa cv. Camarosa) roots with a H2S donor, sodium hydrosulfide (NaHS; 100 μM for 48 h), could induce long-lasting priming effects and tolerance to subsequent exposure to 100mM NaCI or 10% (w/v) PEG-6000 for 7 d. Hydrogen sulfide pretreatment of roots resulted in increased leaf chlorophyll fluorescence, stomatal conductance ...[more]

PMID: 23567865

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Project description:Background and aimsGlobal climate change includes shifts in temperature and precipitation, increases in the frequency and intensity of extreme weather events and sea level rise, which will drastically impact coastal ecosystems. The aim of this study is to quantify salinity tolerance and to identify physiological mechanisms underlying tolerance across wholeplant ontogeny in two widespread native coastal plant species in Hawai'i, Jacquemontia sandwicensis (Convolvulaceae) and Sida fallax (Malvaceae).MethodsAt the seed, seedling, juvenile and mature ontogenetic stages, plants were exposed to high salinity watering treatments. Tolerance was assayed as the performance of stressed compared with control plants using multiple fitness metrics, including germination, survival, growth and reproduction. Potential physiological mechanisms underlying salinity tolerance were measured at each ontogenetic stage, including: photosynthesis and stomatal conductance rates, leaf thickness, leaf mass per area and biomass allocation.Key resultsSalinity tolerance varied between species and across ontogeny but, overall, salinity tolerance increased across ontogeny. For both species, salinity exposure delayed flowering. Physiological and morphological leaf traits shifted across plant ontogeny and were highly plastic in response to salinity. Traits enhancing performance under high salinity varied across ontogeny and between species. For J. sandwicensis, water use efficiency enhanced growth for juvenile plants exposed to high salinity, while chlorophyll content positively influenced plant growth under salinity in the mature stage. For S. fallax, transpiration enhanced plant growth only under low salinity early in ontogeny; high transpiration constrained growth under high salinity across all ontogenetic stages.ConclusionsThat salinity effects vary across ontogenetic stages indicates that demographic consequences of sea level rise and coastal flooding will influence population dynamics in complex ways. Furthermore, even coastal dune plants presumably adapted to tolerate salinity demonstrate reduced ecophysiological performance, growth and reproduction under increased salinity, highlighting the conservation importance of experimental work to better project climate change effects on plants.

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Dataset Information

Hydrogen sulfide induces systemic tolerance to salinity and non-ionic osmotic stress in strawberry plants through modification of reactive species biosynthesis and transcriptional regulation of multiple defence pathways.

Publications

Hydrogen sulfide induces systemic tolerance to salinity and non-ionic osmotic stress in strawberry plants through modification of reactive species biosynthesis and transcriptional regulation of multiple defence pathways.

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