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Developing baculovirus-insect cell expression systems for humanized recombinant glycoprotein production.

ABSTRACT: The baculovirus-insect cell expression system is widely used to produce recombinant glycoproteins for many different biomedical applications. However, due to the fundamental nature of insect glycoprotein processing pathways, this system is typically unable to produce recombinant mammalian glycoproteins with authentic oligosaccharide side chains. This minireview summarizes our current understanding of insect protein glycosylation pathways and our recent efforts to address this problem. These efforts have yielded new insect cell lines and baculoviral vectors that can produce recombinant glycoproteins with humanized oligosaccharide side chains.

SUBMITTER: Jarvis DL

PROVIDER: S-EPMC3641552 | biostudies-other | 2003 May

REPOSITORIES: biostudies-other

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Developing baculovirus-insect cell expression systems for humanized recombinant glycoprotein production.

Jarvis Donald L DL

Virology 20030501 1

The baculovirus-insect cell expression system is widely used to produce recombinant glycoproteins for many different biomedical applications. However, due to the fundamental nature of insect glycoprotein processing pathways, this system is typically unable to produce recombinant mammalian glycoproteins with authentic oligosaccharide side chains. This minireview summarizes our current understanding of insect protein glycosylation pathways and our recent efforts to address this problem. These effo ...[more]

PMID: 12788624

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Expression of recombinant allergen, Der f 1, Der f 2 and Der f 4 using baculovirus-insect cell systems.

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