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Analysis of a replication initiation sequence from the adenosine deaminase region of the mouse genome.

ABSTRACT: A 4-kb HindIII fragment that supported the efficient autonomous replication of plasmid vector pDY-, a replication-defective construct based on Epstein-Barr virus sequences, in human K562 cells was rescued from amplified double-minute chromosomes containing the murine adenosine deaminase locus. Polymerase chain reaction assays of size-fractionated nascent strands demonstrated that replication initiation occurred within the same 1- to 2-kb region of this fragment in autonomously replicating plasmids containing the sequence in either orientation, in double-minute chromosomes, and in the single-copy locus at its normal chromosomal location. The complete sequence of this fragment was determined; it contains a 248-bp polypurine tract and consensus binding site sequences for several putative transcription and replication factors.

SUBMITTER: Virta-Pearlman VJ

PROVIDER: S-EPMC364638 | biostudies-other | 1993 Oct

REPOSITORIES: biostudies-other

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Analysis of a replication initiation sequence from the adenosine deaminase region of the mouse genome.

Virta-Pearlman V J VJ Gunaratne P H PH Chinault A C AC

Molecular and cellular biology 19931001 10

A 4-kb HindIII fragment that supported the efficient autonomous replication of plasmid vector pDY-, a replication-defective construct based on Epstein-Barr virus sequences, in human K562 cells was rescued from amplified double-minute chromosomes containing the murine adenosine deaminase locus. Polymerase chain reaction assays of size-fractionated nascent strands demonstrated that replication initiation occurred within the same 1- to 2-kb region of this fragment in autonomously replicating plasmi ...[more]

PMID: 8413198

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