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¹H, ¹³C and ¹?N backbone and side-chain resonance assignments of Drosophila melanogaster Ssu72.

ABSTRACT: Ssu72 helps regulate transcription and co-transcriptional mRNA processing by dephosphorylating serine residues at the 5th position in the heptad repeats of the C-terminal domain of RNA polymerase II. Here we use multidimensional, multinuclear NMR experiments to assign the backbone and side-chain resonances of the 23 kDa Ssu72 from Drosophila melanogaster in the phosphate-bound state, and use NMR titrations to examine the phosphate-binding properties of three active site mutants.

SUBMITTER: Werner-Allen JW

PROVIDER: S-EPMC3653315 | biostudies-other | 2012 Apr

REPOSITORIES: biostudies-other

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¹H, ¹³C and ¹⁵N backbone and side-chain resonance assignments of Drosophila melanogaster Ssu72.

Werner-Allen Jon W JW Zhou Pei P

Biomolecular NMR assignments 20110706 1

Ssu72 helps regulate transcription and co-transcriptional mRNA processing by dephosphorylating serine residues at the 5th position in the heptad repeats of the C-terminal domain of RNA polymerase II. Here we use multidimensional, multinuclear NMR experiments to assign the backbone and side-chain resonances of the 23 kDa Ssu72 from Drosophila melanogaster in the phosphate-bound state, and use NMR titrations to examine the phosphate-binding properties of three active site mutants. ...[more]

PMID: 21732054

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