Ontology highlight
ABSTRACT:
SUBMITTER: Xie J
PROVIDER: S-EPMC3657831 | biostudies-other | 2013 May
REPOSITORIES: biostudies-other
Proceedings of the National Academy of Sciences of the United States of America 20130423 20
We report here the generation of antibody agonists from intracellular combinatorial libraries that transdifferentiate human stem cells. Antibodies that are agonists for the granulocyte colony stimulating factor receptor were selected from intracellular libraries on the basis of their ability to activate signaling pathways in reporter cells. We used a specialized "near neighbor" approach in which the entire antibody library and its target receptor are cointegrated into the plasma membranes of a p ...[more]