Characterization of a Methanogenic Community within an Algal Fed Anaerobic Digester.
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ABSTRACT:
SUBMITTER: Ellis JT
PROVIDER: S-EPMC3658636 | biostudies-other | 2012
REPOSITORIES: biostudies-other
Publications
Characterization of a Methanogenic Community within an Algal Fed Anaerobic Digester.
Ellis Joshua T JT Tramp Cody C Sims Ronald C RC Miller Charles D CD
ISRN microbiology 20120621
The microbial diversity and metabolic potential of a methanogenic consortium residing in a 3785-liter anaerobic digester, fed with wastewater algae, was analyzed using 454 pyrosequencing technology. DNA was extracted from anaerobic sludge material and used in metagenomic analysis through PCR amplification of the methyl-coenzyme M reductase α subunit (mcrA) gene using primer sets ML, MCR, and ME. The majority of annotated mcrA sequences were assigned taxonomically to the genera Methanosaeta in th ...[more]
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