Unknown

Dataset Information

0

Methodology for labeling proteins and peptides with lead-212 (212Pb).


ABSTRACT: Alpha particles possess an exquisite degree of cytotoxicity when employed for targeted ?-particle therapy (TAT) or radioimmunotherapy (RIT). (212)Pb, which acts as an in vivo generator of the ?-emitting nuclide (212)Bi has shown great promise in pre-clinical studies when used to label the HER2 binding antibody, trastuzumab. Currently, the first RIT clinical trial employing (212)Pb radiolabeled trastuzumab is in progress. This report provides detailed current protocol operations and steps that were generated for use in the clinical trial as well as the relevant pre-clinical experimentation, and describes in detail the labeling of proteins or peptides with (212)Pb as provided via a (224)Ra based generator system.(212)Pb was eluted from the (224)Ra/(212)Pb generator using hydrochloric acid (2M). The generator eluate was evaporated and digested with nitric acid (8M) followed by extraction of the (212)Pb with dilute nitric acid (0.1M). The dilute nitric acid solution of (212)Pb was used to label the immunoconjugate Trastuzumab-TCMC (2-(4-isothiocyanatobenzyl-1,4,7,10-tetraaza-1,4,7,10,tetra-(2-carbamonylmethyl)-cyclododecane) at pH5.5.Elution of (212)Pb from the generator was efficient yielding>90% of available (212)Pb. Trastuzumab-TCMC was efficiently labeled with a radiochemical yield of 94% ± 4% (n=7) by ITLC and an isolated yield of 73% ± 3% (n=7).The results show the feasibility of generating radioimmunoconjugates and peptide conjugates for use as in vivo ? generator systems in the clinic. The technology holds promise in applications involving the treatment of minimal disease such as micrometastases and residual tumor after surgical debulking, hematological cancers, infections, and compartmental cancers, such as ovarian cancer.

SUBMITTER: Baidoo KE 

PROVIDER: S-EPMC3665721 | biostudies-other | 2013 Jul

REPOSITORIES: biostudies-other

altmetric image

Publications

Methodology for labeling proteins and peptides with lead-212 (212Pb).

Baidoo Kwamena E KE   Milenic Diane E DE   Brechbiel Martin W MW  

Nuclear medicine and biology 20130417 5


<h4>Introduction</h4>Alpha particles possess an exquisite degree of cytotoxicity when employed for targeted α-particle therapy (TAT) or radioimmunotherapy (RIT). (212)Pb, which acts as an in vivo generator of the α-emitting nuclide (212)Bi has shown great promise in pre-clinical studies when used to label the HER2 binding antibody, trastuzumab. Currently, the first RIT clinical trial employing (212)Pb radiolabeled trastuzumab is in progress. This report provides detailed current protocol operati  ...[more]

Similar Datasets

| S-EPMC4058400 | biostudies-literature
| S-EPMC4107141 | biostudies-literature
| S-EPMC6776382 | biostudies-literature
| S-EPMC5469783 | biostudies-literature
2023-06-01 | GSE202764 | GEO
| S-EPMC6270137 | biostudies-literature
| S-EPMC3177243 | biostudies-literature
| S-EPMC5488175 | biostudies-literature
| S-EPMC3176724 | biostudies-literature
| S-EPMC3865927 | biostudies-literature