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A new optical leaf-clip meter for simultaneous non-destructive assessment of leaf chlorophyll and epidermal flavonoids.

ABSTRACT: We have characterized a new commercial chlorophyll (Chl) and flavonoid (Flav) meter called Dualex 4 Scientific (Dx4). We compared this device to two other Chl meters, the SPAD-502 and the CCM-200. In addition, Dx4 was compared to the leaf-clip Dualex 3 that measures only epidermal Flav. Dx4 is factory-calibrated to provide a linear response to increasing leaf Chl content in units of µg cm(-2), as opposed to both SPAD-502 and CCM-200 that have a non-linear response to leaf Chl content. Our comparative calibration by Chl extraction confirmed these responses. It seems that the linear response of Dx4 derives from the use of 710 nm as the sampling wavelength for transmittance. The major advantage of Dx4 is its simultaneous assessment of Chl and Flav on the same leaf spot. This allows the generation of the nitrogen balance index (NBI) used for crop surveys and nitrogen nutrition management. The Dx4 leaf clip, that incorporates a GPS receiver, can be useful for non-destructive estimation of leaf Chl and Flav contents for ecophysiological research and ground truthing of remote sensing of vegetation. In this work, we also propose a consensus equation for the transformation of SPAD units into leaf Chl content, for general use.

SUBMITTER: Cerovic ZG

PROVIDER: S-EPMC3666089 | biostudies-other | 2012 Nov

REPOSITORIES: biostudies-other

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A new optical leaf-clip meter for simultaneous non-destructive assessment of leaf chlorophyll and epidermal flavonoids.

Cerovic Zoran G ZG Masdoumier Guillaume G Ghozlen Naïma Ben NB Latouche Gwendal G

Physiologia plantarum 20120606 3

We have characterized a new commercial chlorophyll (Chl) and flavonoid (Flav) meter called Dualex 4 Scientific (Dx4). We compared this device to two other Chl meters, the SPAD-502 and the CCM-200. In addition, Dx4 was compared to the leaf-clip Dualex 3 that measures only epidermal Flav. Dx4 is factory-calibrated to provide a linear response to increasing leaf Chl content in units of µg cm(-2), as opposed to both SPAD-502 and CCM-200 that have a non-linear response to leaf Chl content. Our compar ...[more]

PMID: 22568678

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Project description:Rapid and non-destructive diagnostic tools to accurately assess crop nitrogen nutrition index (NNI) are imperative for improving crop nitrogen (N) diagnosis and sustaining crop production. This study was aimed to develop the relationships among NNI, leaf N gradient, chlorophyll meter (CM) readings gradient, and positional differences chlorophyll meter index [PDCMI, the ratio of CM readings between different leaf layers (LLs) of crop canopy] and to validate the accuracy and stability of these relationships across the different LLs, years, sites, and cultivars. Six multi-N rates (0-320 kg ha-1) field experiments were conducted with four summer maize cultivars (Zhengdan958, Denghai605, Xundan20, and Denghai661) at two different sites located in China. Six summer maize plants per plot were harvested at each sampling stage to assess NNI, leaf N concentration and CM readings of different LLs during the vegetative growth period. The results showed that the leaf N gradient, CM readings gradient and PDCMI of different LLs decreased, while the NNI values increased with increasing N supply. The leaf N gradient and CM readings gradient increased gradually from top to bottom of the canopy and CM readings of the bottom LL were more sensitive to changes in plant N concentration. The significantly positive relationship between NNI and CM readings of different LLs (LL1 to LL3) was observed, yet these relationships varied across the years. In contrast, the relationships between NNI and PDCMI of different LLs (LL1 to LL3) were significantly negative. The strongest relationship between PDCMI and NNI which was stable across the cultivars and years was observed for PDCMI1-3 (NNI = -5.74 × PDCMI1-3+1.5, R2 = 0.76**). Additionally, the models developed in this study were validated with the data acquired from two independent experiments to assess their accuracy of prediction. The root mean square error value of 0.1 indicated that the most accurate and robust relationship was observed between PDCMI1-3 and NNI. The projected results would help to develop a simple, non-destructive and reliable approach to accurately assess the crop N status for precisely managing N application during the growth period of summer maize crop.

Project description:Reliability and economic assessment of the Multiplex® optical sensor employed for non-destructive grape parameters estimates was evaluated in 2017 against a wet chemistry approach in mature vineyards including five cultivars (two whites, two red and one pink colored) assuring a broad range of variation in both technological and phenolic maturity parameters. Among the five Multiplex (Mx) indices evaluated (SFR_R, FLAV, FLAV-UV, ANTH_RG and FERARI) in all cultivars with the exception of Barbera SFR_R showed a significant and linear correlation with total soluble solids (TSS) for TSS ≥ 10 °Brix. Conversely, no significant correlation was found between FLAV and total flavonols concentration, whereas a significant linear correlation was found in Barbera (R2 = 0.66) and Ervi (R2 = 0.63) when the FLAV index was replaced with the FLAV_UV index. Within each cultivar, both ANTH_RG and FERARI showed close correlations with total anthocyanins concentration determined by wet chemistry although under different model shapes. Expressing berry color accumulation on a per skin mass basis rather than for whole berry mass basis, allowed for better separation of behavior of single cultivars and improved accuracy of model fitting for the combination of Barbera and ANTH_RG. A strict linear correlation was always found, within each index, for Mx readings taken on the two opposite sides of the same cluster, implying no significant within-cluster differences in sugar, color and flavonol concentrations. Economic assessment of Mx by means of the Net Present Value (NPV) approach showed that Mx is economically viable for a two hectare vineyard cultivating three red grape varieties (90 samples per year) if its lifetime is at least 7 years. Conversely, if only two red varieties are grown Mx should be used at least 11 years to make it economic suitable. Bigger properties growing a higher number of red varieties are the more interested in Mx as the expected NPV assumes positive values with a Mx usage of minimum 3 years.

Dataset Information

A new optical leaf-clip meter for simultaneous non-destructive assessment of leaf chlorophyll and epidermal flavonoids.

Publications

A new optical leaf-clip meter for simultaneous non-destructive assessment of leaf chlorophyll and epidermal flavonoids.

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