Rapid and simultaneous detection of blaKPC and blaNDM by use of multiplex real-time PCR.
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ABSTRACT:
SUBMITTER: Milillo M
PROVIDER: S-EPMC3666810 | biostudies-other | 2013 Apr
REPOSITORIES: biostudies-other
Publications
Rapid and simultaneous detection of blaKPC and blaNDM by use of multiplex real-time PCR.
Milillo Michael M Kwak Yoon I YI Snesrud Erik E Waterman Paige E PE Lesho Emil E McGann Patrick P
Journal of clinical microbiology 20130116 4
The increasing incidence of carbapenem nonsusceptibility among clinically important species is of global concern. Identification of the molecular mechanisms underlying carbapenem nonsusceptibility is critical for epidemiological investigations. In this report, we describe a real-time PCR-based assay capable of simultaneously detecting blaKPC and blaNDM, two of the most important carbapenemases, directly from culture in less than 90 min. The assay was validated with blaKPC- and blaNDM-carrying cl ...[more]
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