Unknown

Dataset Information

0

Intracellular temperature mapping with fluorescence-assisted photoacoustic-thermometry.


ABSTRACT: Measuring intracellular temperature is critical to understanding many cellular functions but still remains challenging. Here, we present a technique-fluorescence-assisted photoacoustic thermometry (FAPT)-for intracellular temperature mapping applications. To demonstrate FAPT, we monitored the intracellular temperature distribution of HeLa cells with sub-degree (0.7 °C) temperature resolution and sub-micron (0.23 μm) spatial resolution at a sampling rate of 1 kHz. Compared to traditional fluorescence-based methods, FAPT features the unique capability of transforming a regular fluorescence probe into a concentration- and excitation-independent temperature sensor, bringing a large collection of commercially available generic fluorescent probes into the realm of intracellular temperature sensing.

SUBMITTER: Gao L 

PROVIDER: S-EPMC3669113 | biostudies-other | 2013 May

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC3293419 | biostudies-literature
| S-EPMC6210794 | biostudies-literature
| S-EPMC7956715 | biostudies-literature
| S-EPMC7229289 | biostudies-literature
| S-EPMC4333297 | biostudies-literature
| S-EPMC6613656 | biostudies-literature
| S-EPMC6013924 | biostudies-literature
| S-EPMC4366424 | biostudies-literature
| S-EPMC5799881 | biostudies-literature
| S-EPMC10862394 | biostudies-literature