A random motility assay based on image correlation spectroscopy.
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ABSTRACT:
SUBMITTER: Prummer M
PROVIDER: S-EPMC3672868 | biostudies-other | 2013 Jun
REPOSITORIES: biostudies-other
Publications
A random motility assay based on image correlation spectroscopy.
Prummer Michael M Kling Dorothee D Trefzer Vanessa V Enderle Thilo T Zoffmann Sannah S Prunotto Marco M
Biophysical journal 20130601 11
We demonstrate the random motility (RAMOT) assay based on image correlation spectroscopy for the automated, label-free, high-throughput characterization of random cell migration. The approach is complementary to traditional migration assays, which determine only the collective net motility in a particular direction. The RAMOT assay is less demanding on image quality compared to single-cell tracking, does not require cell identification or trajectory reconstruction, and performs well on live-cell ...[more]
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