Ontology highlight
ABSTRACT:
SUBMITTER: Lauterbach MA
PROVIDER: S-EPMC3689173 | biostudies-other | 2013
REPOSITORIES: biostudies-other
Scientific reports 20130101
Stimulated Emission Depletion (STED) microscopy enables superresolution imaging of fluorescently marked nano-structures in vivo. Biological investigations are often hindered by the difficulty of relating super-resolved structures to other non-labeled features. Here we demonstrate that the similarity in optical design of Spiral Phase Contrast (SPC) and STED microscopes allows straightforward implementation of a phase contrast channel into a STED microscope in widefield and scanning modes. This me ...[more]