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Microsecond dynamics of an unfolded protein by a line confocal tracking of single molecule fluorescence.

ABSTRACT: We present a new method for high speed tracking of fluorescence time series from single proteins. The method uses a fast sample flow and a modified confocal microscopy, line confocal microscopy, and achieves the time resolution of less than 20??s. The obtained time series from the B domain of protein A labeled with donor and acceptor fluorophores suggest conformational heterogeneity and dynamic fluctuations in the unfolded state.

SUBMITTER: Oikawa H 

PROVIDER: S-EPMC3701896 | biostudies-other | 2013

REPOSITORIES: biostudies-other

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Microsecond dynamics of an unfolded protein by a line confocal tracking of single molecule fluorescence.

Oikawa Hiroyuki H   Suzuki Yuta Y   Saito Masataka M   Kamagata Kiyoto K   Arai Munehito M   Takahashi Satoshi S  

Scientific reports 20130101

We present a new method for high speed tracking of fluorescence time series from single proteins. The method uses a fast sample flow and a modified confocal microscopy, line confocal microscopy, and achieves the time resolution of less than 20 μs. The obtained time series from the B domain of protein A labeled with donor and acceptor fluorophores suggest conformational heterogeneity and dynamic fluctuations in the unfolded state. ...[more]