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High-throughput sequencing of B- and T-lymphocyte antigen receptors in hematology.

ABSTRACT: Application of high-throughput DNA sequencing to the analysis of B- and T-lymphocyte antigen receptors has great potential for improving the monitoring of lymphoid malignancies, assessing immune reconstitution after hematopoietic cell transplantation, and characterizing the composition of lymphocyte repertoires. Current technology can define the number and frequency of immunoglobulin heavy, T-cell receptor (TCR)?, TCR?, or TCR? chains expressed in a population of lymphocytes; techniques for determining the number of antigen receptor heterodimers, such as TCR?? pairs, expressed in the population are under development.

SUBMITTER: Warren EH

PROVIDER: S-EPMC3701903 | biostudies-other | 2013 Jul

REPOSITORIES: biostudies-other

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High-throughput sequencing of B- and T-lymphocyte antigen receptors in hematology.

Warren Edus H EH Matsen Frederick A FA Chou Jeffrey J

Blood 20130508 1

Application of high-throughput DNA sequencing to the analysis of B- and T-lymphocyte antigen receptors has great potential for improving the monitoring of lymphoid malignancies, assessing immune reconstitution after hematopoietic cell transplantation, and characterizing the composition of lymphocyte repertoires. Current technology can define the number and frequency of immunoglobulin heavy, T-cell receptor (TCR)α, TCRβ, or TCRγ chains expressed in a population of lymphocytes; techniques for dete ...[more]

PMID: 23656731

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