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Intratumoral DNA electroporation induces anti-tumor immunity and tumor regression.


ABSTRACT: In situ expression of a foreign antigen and an immune-modulating cytokine by intratumoral DNA electroporation was tested as a cancer therapy regimen. Transgene expression in the tumors was sustained for 2-3 weeks after intratumoral electroporation with mammalian expression plasmid containing firefly luciferase cDNA. Electroporation with cDNA encoding tetanus toxin fragment C (TetC) induced tetanus toxin-binding antibody, demonstrating immune recognition of the transgene product. Intratumoral electroporation with TetC and IL-12 cDNA after mice were treated with CD25 mAb to remove regulatory T cells induced IFN-gamma producing T-cell response to tumor-associated antigen, heavy inflammatory infiltration, regression of established tumors and immune memory to protect mice from repeated tumor challenge. Intratumoral expression of immune-modulating molecules may be most suitable in the neoadjuvant setting to enhance the therapeutic efficacy and provide long-term protection.

SUBMITTER: Radkevich-Brown O 

PROVIDER: S-EPMC3702174 | biostudies-other | 2010 Mar

REPOSITORIES: biostudies-other

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Intratumoral DNA electroporation induces anti-tumor immunity and tumor regression.

Radkevich-Brown Olga O   Piechocki Marie P MP   Back Jessica B JB   Weise Amy M AM   Pilon-Thomas Shari S   Wei Wei-Zen WZ  

Cancer immunology, immunotherapy : CII 20100301 3


In situ expression of a foreign antigen and an immune-modulating cytokine by intratumoral DNA electroporation was tested as a cancer therapy regimen. Transgene expression in the tumors was sustained for 2-3 weeks after intratumoral electroporation with mammalian expression plasmid containing firefly luciferase cDNA. Electroporation with cDNA encoding tetanus toxin fragment C (TetC) induced tetanus toxin-binding antibody, demonstrating immune recognition of the transgene product. Intratumoral ele  ...[more]

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