Unknown

Dataset Information

0

Secondary structure of the HIV reverse transcription initiation complex by NMR.


ABSTRACT: Initiation of reverse transcription of genomic RNA is a key early step in replication of the human immunodeficiency virus (HIV) upon infection of a host cell. Viral reverse transcriptase initiates from a specific RNA-RNA complex formed between a host transfer RNA (tRNA(Lys)(3)) and a region at the 5' end of genomic RNA; the 3' end of the tRNA acts as a primer for reverse transcription of genomic RNA. We report here the secondary structure of the HIV genomic RNA-human tRNA(Lys)(3) initiation complex using heteronuclear nuclear magnetic resonance methods. We show that both RNAs undergo large-scale conformational changes upon complex formation. Formation of the 18-bp primer helix with the 3' end of tRNA(Lys)(3) drives large conformational rearrangements of the tRNA at the 5' end while maintaining the anticodon loop for potential loop-loop interactions. HIV RNA forms an intramolecular helix adjacent to the intermolecular primer helix. This helix, which must be broken by reverse transcription, likely acts as a kinetic block to reverse transcription.

SUBMITTER: Puglisi EV 

PROVIDER: S-EPMC3710119 | biostudies-other | 2011 Jul

REPOSITORIES: biostudies-other

altmetric image

Publications

Secondary structure of the HIV reverse transcription initiation complex by NMR.

Puglisi Elisabetta Viani EV   Puglisi Joseph D JD  

Journal of molecular biology 20110701 5


Initiation of reverse transcription of genomic RNA is a key early step in replication of the human immunodeficiency virus (HIV) upon infection of a host cell. Viral reverse transcriptase initiates from a specific RNA-RNA complex formed between a host transfer RNA (tRNA(Lys)(3)) and a region at the 5' end of genomic RNA; the 3' end of the tRNA acts as a primer for reverse transcription of genomic RNA. We report here the secondary structure of the HIV genomic RNA-human tRNA(Lys)(3) initiation comp  ...[more]

Similar Datasets

| S-EPMC3058889 | biostudies-literature
| S-EPMC5934294 | biostudies-literature
| S-EPMC5218889 | biostudies-literature
| S-EPMC7387199 | biostudies-literature
| S-EPMC5066621 | biostudies-literature
| S-EPMC4668388 | biostudies-literature
| S-EPMC6289658 | biostudies-literature
| S-EPMC3386134 | biostudies-literature
| S-EPMC2775702 | biostudies-literature
2017-03-01 | GSE95483 | GEO