Femtosecond laser processing by using patterned vector optical fields.
Ontology highlight
ABSTRACT:
SUBMITTER: Lou K
PROVIDER: S-EPMC3722566 | biostudies-other | 2013
REPOSITORIES: biostudies-other
Similar Datasets
Project description:We report on a new, single-step and scalable method to fabricate highly ordered, multi-directional and complex surface structures that mimic the unique morphological features of certain species found in nature. Biomimetic surface structuring was realized by exploiting the unique and versatile angular profile and the electric field symmetry of cylindrical vector (CV) femtosecond (fs) laser beams. It is shown that, highly controllable, periodic structures exhibiting sizes at nano-, micro- and dual- micro/nano scales can be directly written on Ni upon line and large area scanning with radial and azimuthal polarization beams. Depending on the irradiation conditions, new complex multi-directional nanostructures, inspired by the Shark's skin morphology, as well as superhydrophobic dual-scale structures mimicking the Lotus' leaf water repellent properties can be attained. It is concluded that the versatility and features variations of structures formed is by far superior to those obtained via laser processing with linearly polarized beams. More important, by exploiting the capabilities offered by fs CV fields, the present technique can be further extended to fabricate even more complex and unconventional structures. We believe that our approach provides a new concept in laser materials processing, which can be further exploited for expanding the breadth and novelty of applications.
| S-EPMC5361190 | biostudies-literature
Project description:The major goal of this study was to create easy-to-use, reusable substrates capable of storing any peptides or bioactive molecules for a desired period of time until cells uptake them without the need for bioactive molecule or peptide-specific techniques. Nanopore arrays of uniform size and distribution were machined into fused silica substrates using femtosecond laser ablation and loaded with peptides by simple adsorption. The nanopore substrates were validated by examining the effect of N-acetyl-seryl-aspartyl-lysyl-proline (Ac-SDKP) loaded nanopores on macrophage phagocytosis and intracellular production of reactive oxygen species (ROS) with and without the pro-inflammatory lipopolysaccharide (LPS). Our results demonstrated that nanopores were generated in a uniform array fashion. Ac-SDKP peptides were stably stored in nanopores and internalized by macrophages. Significant reductions in ROS production and phagocytosis in macrophages were observed over control substrates, even in combination with LPS stimulation, indicating that loading Ac-SDKP peptides in pores significantly improved the anti-inflammatory effects.This team of scientists intended to create easy-to-use, reusable substrates for storing peptides or bioactive molecules for a desired period of time before cellular uptake occurs, and without the need for bioactive molecule or peptide-specific techniques. They demonstrate the successful generation of nanopores in a uniform array that stably stores Ac-SDKP peptides in the nanopores. When peptides were internalized by macrophages, significant reductions in ROS production and phagocytosis were observed, indicating improved anti-inflammatory effects.
| S-EPMC3877160 | biostudies-literature
Project description:Optical diffusers are widely used in filament lamps, imaging systems, display technologies, lasers, and Light Emitting Diodes (LEDs). Here, a method for the fabrication of optical diffusers through femtosecond laser machining is demonstrated. Float glass surfaces were ablated with femtosecond laser light to form nanoscale ripples with dimensions comparable to the wavelength of visible light. These structures produce highly efficient and wide field of view diffusers. The machined patterns altered the average surface roughness, with the majority of particles in the range of a few hundred nanometers. The optical diffusion characteristic and a maximum diffusion angle of near 172° was achieved with optimum machining parameters. The transmission performance of the diffusers was measured to be ∼30% across the visible spectrum. The demonstrated technique has potential for producing low-cost large area optical devices. The process benefits from the flexibility of the laser writing method and enables the production of custom optical diffusers.
| S-EPMC6112377 | biostudies-literature
Project description:We experimentally investigate generation of nitrogen molecular ion (N2+) lasers with two femtosecond laser pulses at different wavelengths. The first pulse serves as the pump which ionizes the nitrogen molecules and excites the molecular ions to excited electronic states. The second pulse serves as the probe which leads to stimulated emission from the excited molecular ions. We observe that changing the angle between the polarization directions of the two pulses gives rise to elliptically polarized N2+ laser fields, which is interpreted as a result of strong birefringence of the gain medium near the wavelengths of the N2+ laser.
| S-EPMC4757838 | biostudies-other
Project description:The emergence of high-powered femtosecond lasers presents the opportunity for large volume processing inside of transparent materials, wherein a myriad of nonlinear optical and aberration effects typically convolves to distort the focused beam shape. In this paper, convex and concave conical phase fronts were imposed on femtosecond laser beams and focussed into wide-bandgap glass to generate a vortex beam with tuneable Gaussian-Bessel features offset from the focal plane. The influence of Kerr lensing, plasma defocussing, and surface aberration on the conical phase front shaping were examined over low to high pulse energy delivery and for shallow to deep processing tested to 2.5 mm focussing depth. By isolating the underlying processes, the results demonstrate how conical beams can systematically manipulate the degree of nonlinear interaction and surface aberration to facilitate a controllable inhibition or enhancement of Kerr lensing, plasma defocussing, and surface aberration effects. In this way, long and uniform filament tracks have been generated over shallow to deep focussing by harnessing surface aberration and conical beam shaping without the destabilizing Kerr lensing and plasma defocussing effects. A facile means for compressing and stretching of the focal interaction volume is presented for controlling the three-dimensional micro- and nano-structuring of transparent materials.
| S-EPMC7726100 | biostudies-literature
Project description:Airway organoids derived from adult murine epithelial cells represent a complex 3D in vitro system mimicking the airway epithelial tissue’s native cell composition and physiological properties. In combination with a precise damage induction via femtosecond laser-based nanosurgery, this model allows for the examination of intra- and intercellular dynamics in the course of repair processes with a high spatio-temporal resolution, which can hardly be reached using in vivo approaches. For characterization of the organoids’ response to single or multiple-cell ablation, we first analyzed overall organoid survival and found that airway organoids were capable of efficiently repairing damage induced by femtosecond laser-based ablation of a single to ten cells within 24 h. An EdU staining assay further revealed a steady proliferative potential of airway organoid cells. Especially in the case of ablation of five cells, proliferation was enhanced within the first four hours upon damage induction, whereas ablation of ten cells was followed by a slight decrease in proliferation within this time frame. Analyzing individual trajectories of single cells within airway organoids, we found an increased migratory behavior in cells within close proximity to the ablation site following the ablation of ten, but not five cells. Bulk RNA sequencing and subsequent enrichment analysis revealed the differential expression of sets of genes involved in the regulation of epithelial repair, distinct signaling pathway activities such as Notch signaling, as well as cell migration after laser-based ablation. Together, our findings demonstrate that organoid repair upon ablation of ten cells involves the same key processes by which native airway epithelial wound healing is regulated. This marks the herein presented in vitro damage model suitable to study repair processes following acute airway injury, thereby posing a novel approach to gain insights into the mechanisms driving epithelial repair on a single-cell level.
2023-08-29 | GSE241237 | GEO
Project description:Polarisation of light is a powerful and widely used degree of freedom to encode information, both in classical and quantum applications. In particular, quantum information technologies based on photons are being revolutionised by the use of integrated photonic circuits. It is therefore very important to be able to manipulate the polarisation of photons in such circuits. We experimentally demonstrate the fabrication by femtosecond laser micromachining of components such as polarisation insensitive and polarising directional couplers, operating at 1550 nm wavelength, where the two opposite behaviours are achieved just by controlling the geometric layout of the photonic circuits, being the waveguides fabricated with the same irradiation recipe. We expect to employ this approach in complex integrated photonic devices, capable of a full control of the photons polarisation for quantum cryptography, quantum computation and quantum teleportation experiments.
| S-EPMC5596026 | biostudies-literature
Project description:We have developed simple and cost-effective surface-enhanced Raman scattering (SERS) substrates for the trace detection of pesticide (thiram and thiabendazole) and dye (methylene blue and Nile blue) molecules. Surface patterns (micro/nanostructures) on silicon (Si) substrates were fabricated using the technique of femtosecond (fs) laser ablation in ambient air. Different surface patterns were achieved by tuning the number of laser pulses per unit area (4200, 8400, 42 000, and 84 000 pulses per mm2) on Si. Subsequently, chemically synthesized gold (Au) nanostars were embedded in these laser-patterned areas of Si to achieve a plasmonic active hybrid SERS substrate. Further, the SERS performance of the as-prepared Au nanostar embedded Si substrates were tested with different probe molecules. The as-prepared substrates allowed us to detect a minimum concentration of 0.1 ppm in the case of thiram, 1 ppm in the case of thiabendazole (TBZ), 1.6 ppb in the case of methylene blue (MB), and 1.8 ppb in case of Nile blue (NB). All these were achieved using a simple, field-deployable, portable Raman spectrometer. Additionally, the optimized SERS substrate demonstrated ∼21 times higher SERS enhancement than the Au nanostar embedded plain Si substrate. Furthermore, the optimized SERS platform was utilized to detect a mixture of dyes (MB + NB) and pesticides (thiram + TBZ). The possible reasons for the observed additional enhancement are elucidated.
| S-EPMC9844677 | biostudies-literature
Project description:Ultrafast injection-locked amplification is achieved by sending femtosecond supercontinuum pulses into a polymeric thin film coated on a distributed feedback (DFB) microcavity consisting of chirped gratings. The spatial variation of the grating period led to the resonance of the DFB microcavity at different wavelengths for injection at different locations. This enables convenient and continuous tuning of the amplification spectrum by displacing the grating structures. The large area of the grating structures enabled large tuning range. The amplified spectrum can be continuously tuned from 545 to 580 nm through sliding the grating structures by about 3.5 mm. Sub-1 ps lifetime has been measured for the amplification process with a net amplification factor as large as 33. Injection locking enabled high-quality control of the divergence and transverse mode of the output laser beam.
| S-EPMC6648656 | biostudies-literature
Project description:Multilayered microfluidic channels integrated with functional microcomponents are the general trend of future biochips, which is similar to the history of Si-integrated circuits from the planer to the three-dimensional (3D) configuration, since they offer miniaturization while increasing the integration degree and diversifying the applications in the reaction, catalysis, and cell cultures. In this paper, an optimized hybrid processing technology is proposed to create true multilayered microchips, by which "all-in-one" 3D microchips can be fabricated with a successive procedure of 3D glass micromachining by femtosecond-laser-assisted wet etching (FLAE) and the integration of microcomponents into the fabricated microchannels by two-photon polymerization (TPP). To create the multilayered microchannels at different depths in glass substrates (the top layer was embedded at 200 μm below the surface, and the underlying layers were constructed with a 200-μm spacing) with high uniformity and quality, the laser power density (13~16.9 TW/cm2) was optimized to fabricate different layers. To simultaneously complete the etching of each layer, which is also important to ensure the high uniformity, the control layers (nonlaser exposed regions) were prepared at the upper ends of the longitudinal channels. Solvents with different dyes were used to verify that each layer was isolated from the others. The high-quality integration was ensured by quantitatively investigating the experimental conditions in TPP, including the prebaking time (18~40 h), laser power density (2.52~2.94 TW/cm2) and developing time (0.8~4 h), all of which were optimized for each channel formed at different depths. Finally, the eight-layered microfluidic channels integrated with polymer microstructures were successfully fabricated to demonstrate the unique capability of this hybrid technique.
| S-EPMC6500790 | biostudies-literature