Ontology highlight
ABSTRACT:
SUBMITTER: Liu SL
PROVIDER: S-EPMC3746204 | biostudies-other | 2013
REPOSITORIES: biostudies-other
Liu Shu-Lin SL Li Jicun J Zhang Zhi-Ling ZL Wang Zhi-Gang ZG Tian Zhi-Quan ZQ Wang Guo-Ping GP Pang Dai-Wen DW
Scientific reports 20130101
We report a non-iterative localization algorithm that utilizes the scaling of a three-dimensional (3D) image in the axial direction and focuses on evaluating the radial symmetry center of the scaled image to achieve the desired single-particle localization. Using this approach, we analyzed simulated 3D particle images by wide-field microscopy and confocal microscopy respectively, and the 3D trajectory of quantum dots (QDs)-labeled influenza virus in live cells. Both applications indicate that th ...[more]