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Hyperspectral fluorescence microfluidic (HFM) microscopy.


ABSTRACT: We present an imaging system that collects hyperspectral images of cells travelling through a microfluidic channel. Using a single monochrome camera and a linear variable bandpass filter (LVF), the system captures a bright field image and a set of hyperspectral fluorescence images for each cell. While the bandwidth of the LVF is 20 nm, we have demonstrated that we can determine the peak wavelength of a fluorescent object's emission spectrum with an accuracy of below 3 nm. In addition, we have used this system to capture fluorescence spectra of individual spatially resolved cellular organelles and to spectrally resolve multiple fluorophores in individual cells.

SUBMITTER: Di Caprio G 

PROVIDER: S-EPMC3756569 | biostudies-other | 2013

REPOSITORIES: biostudies-other

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