Unknown

Dataset Information

0

Hyperspectral fluorescence microfluidic (HFM) microscopy.


ABSTRACT: We present an imaging system that collects hyperspectral images of cells travelling through a microfluidic channel. Using a single monochrome camera and a linear variable bandpass filter (LVF), the system captures a bright field image and a set of hyperspectral fluorescence images for each cell. While the bandwidth of the LVF is 20 nm, we have demonstrated that we can determine the peak wavelength of a fluorescent object's emission spectrum with an accuracy of below 3 nm. In addition, we have used this system to capture fluorescence spectra of individual spatially resolved cellular organelles and to spectrally resolve multiple fluorophores in individual cells.

SUBMITTER: Di Caprio G 

PROVIDER: S-EPMC3756569 | biostudies-other | 2013

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC6800214 | biostudies-literature
| S-EPMC4585673 | biostudies-literature
| S-EPMC8707055 | biostudies-literature
| S-EPMC4840377 | biostudies-literature
| S-EPMC4569691 | biostudies-literature
| S-EPMC6028425 | biostudies-literature
| S-EPMC9617892 | biostudies-literature
| S-EPMC7608807 | biostudies-literature
| S-EPMC8205435 | biostudies-literature
| S-EPMC10802330 | biostudies-literature