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Spatial Light Interference Microscopy (SLIM) using twisted-nematic liquid-crystal modulation.


ABSTRACT: We report the use of a twisted nematic liquid-crystal spatial light modulator (TNLC-SLM) for quantitative phase imaging. The experimental setup is a new implementation of the SLIM principle, which is a phase shifting, white light method for quantitative phase imaging. The approach is based on switching between the phase and amplitude modulation modes of the SLM. Our system is able to deliver a 0.99 nm spatial and 1.33 nm temporal pathlength sensitivity while retaining the optical transverse resolution. The system is implemented as an additional module mounted to a conventional microscope, which makes the system very easy to deploy and integrate with other imaging modalities.

SUBMITTER: Nguyen TH 

PROVIDER: S-EPMC3771828 | biostudies-other | 2013

REPOSITORIES: biostudies-other

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