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Heritable gene knockout in Caenorhabditis elegans by direct injection of Cas9-sgRNA ribonucleoproteins.

ABSTRACT: We present a novel method of targeted gene disruption that involves direct injection of recombinant Cas9 protein complexed with guide RNA into the gonad of the nematode Caenorhabditis elegans. Biallelic mutants were recovered among the F1 progeny, demonstrating the high efficiency of this method.

SUBMITTER: Cho SW

PROVIDER: S-EPMC3813847 | biostudies-other | 2013 Nov

REPOSITORIES: biostudies-other

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Heritable gene knockout in Caenorhabditis elegans by direct injection of Cas9-sgRNA ribonucleoproteins.

Cho Seung Woo SW Lee Jihyun J Carroll Dana D Kim Jin-Soo JS Lee Junho J

Genetics 20130826 3

We present a novel method of targeted gene disruption that involves direct injection of recombinant Cas9 protein complexed with guide RNA into the gonad of the nematode Caenorhabditis elegans. Biallelic mutants were recovered among the F1 progeny, demonstrating the high efficiency of this method. ...[more]

PMID: 23979576

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