Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

How to decrease the viscosity of suspension with the second fluid and nanoparticles?

ABSTRACT: According to recent research reports, addition of small amounts of a secondary fluid to a suspension could dramatically increase viscosity of suspension. Results of this study indicate another interesting behavior that the secondary fluid could form a thin hydrophobic membrane around particle surface and significantly decrease the viscosity and yield stress of the suspension. To enhance the surface hydrophobicity, hydrophobic nanoparticles (nano-CaCO3) were added to the hydrophobic membrane of particles to improve the surface roughness, and to generate composite particles having a hierarchical structure similar to the micromorphology of lotus leaf. This composite particle has a higher contact angle, and the suspension of composite particles has a lower viscosity and a lower yield stress.

SUBMITTER: Xu M

PROVIDER: S-EPMC3817430 | biostudies-other | 2013

REPOSITORIES: biostudies-other

ACCESS DATA

Json Xml

Publications

How to decrease the viscosity of suspension with the second fluid and nanoparticles?

Xu Menghan M Liu Haifeng H Zhao Hui H Li Weifeng W

Scientific reports 20131105

According to recent research reports, addition of small amounts of a secondary fluid to a suspension could dramatically increase viscosity of suspension. Results of this study indicate another interesting behavior that the secondary fluid could form a thin hydrophobic membrane around particle surface and significantly decrease the viscosity and yield stress of the suspension. To enhance the surface hydrophobicity, hydrophobic nanoparticles (nano-CaCO3) were added to the hydrophobic membrane of p ...[more]

PMID: 24190438

Similar Datasets

Multiple Effects of the Second Fluid on Suspension Viscosity.

Project description:Previous research has shown that adding a small amount of a second immiscible fluid to particulate suspension can result in a significant influence on viscosity. In this study, the effects of the second fluid addition over a small dosage range on the rheological properties of particle suspension were investigated. As the dosage of the second fluid was increased, the viscosity and yield stress initially decreased then increased and finally decreased again. The microstructure of the suspension was observed using a confocal laser scanning microscope (CLSM) and showed three different states with the increasing dosage of the second fluid: a dispersive sate, cluster state and cell state in sequence. The presence of these states interpreted the non-monotonic trend of viscosity and yield stress in the suspensions.

| S-EPMC4629204 | biostudies-literature

Counterintuitive effect of extracellular fluid viscosity on enhancing motility and metastasis

Project description:Cells respond to physical stimuli, such as stiffness, fluid shear stress and hydraulic pressure. Extracellular fluid viscosity is a key physical cue that varies under physiological and pathological conditions, such as cancer. However, its impact on cancer biology and the mechanism by which cells sense and respond to changes in viscosity is unknown. We demonstrate that elevated viscosity counterintuitively increases the motility of various cell types on two-dimensional (2D) surfaces, in confinement, and cell dissemination from 3D tumor spheroids. Increased mechanical loading imposed by elevated viscosity induces an Actin Related Protein 2/3 (Arp2/3) complex-dependent dense actin network, which enhances Na+/H+ exchanger 1 (NHE1) polarization via its actin-binding partner ezrin. NHE1 promotes cell swelling and increased membrane tension which, in turn, activates transient receptor potential cation vanilloid 4 (TRPV4) and mediates calcium influx, leading to increased RhoA-dependent cell contractility. The coordinated action of actin remodeling/dynamics, NHE1-mediated swelling and RhoA-based contractility facilitates enhanced motility at elevated viscosities. Breast cancer cells pre-exposed to elevated viscosity acquire TRPV4-dependent mechanical memory via transcriptional control of the Hippo pathway, leading to increased migration in zebrafish, extravasation in chick embryos and lung metastasis in mice. Cumulatively, extracellular viscosity is a physical cue that regulates both short- and long-term cellular processes with pathophysiological relevance to cancer biology.

2022-07-27 | GSE203651 | GEO

Extracellular fluid viscosity enhances cell migration and cancer dissemination.

Project description:Cells respond to physical stimuli, such as stiffness1, fluid shear stress2 and hydraulic pressure3,4. Extracellular fluid viscosity is a key physical cue that varies under physiological and pathological conditions, such as cancer5. However, its influence on cancer biology and the mechanism by which cells sense and respond to changes in viscosity are unknown. Here we demonstrate that elevated viscosity counterintuitively increases the motility of various cell types on two-dimensional surfaces and in confinement, and increases cell dissemination from three-dimensional tumour spheroids. Increased mechanical loading imposed by elevated viscosity induces an actin-related protein 2/3 (ARP2/3)-complex-dependent dense actin network, which enhances Na+/H+ exchanger 1 (NHE1) polarization through its actin-binding partner ezrin. NHE1 promotes cell swelling and increased membrane tension, which, in turn, activates transient receptor potential cation vanilloid 4 (TRPV4) and mediates calcium influx, leading to increased RHOA-dependent cell contractility. The coordinated action of actin remodelling/dynamics, NHE1-mediated swelling and RHOA-based contractility facilitates enhanced motility at elevated viscosities. Breast cancer cells pre-exposed to elevated viscosity acquire TRPV4-dependent mechanical memory through transcriptional control of the Hippo pathway, leading to increased migration in zebrafish, extravasation in chick embryos and lung colonization in mice. Cumulatively, extracellular viscosity is a physical cue that regulates both short- and long-term cellular processes with pathophysiological relevance to cancer biology.

| S-EPMC9646524 | biostudies-literature

Odd viscosity-induced Hall-like transport of an active chiral fluid.

Project description:Broken time-reversal and parity symmetries in active spinner fluids imply a nondissipative "odd viscosity," engendering phenomena unattainable in traditional passive or active fluids. Here we show that the odd viscosity itself can lead to a Hall-like transport when the active chiral fluid flows through a quenched matrix of obstacles, reminiscent of the anomalous Hall effect. The Hall-like velocity depends significantly on the spinner activity and longitudinal flow due to the interplay between odd viscosity and spinner-obstacle collisions. Our findings underscore the importance of odd viscosity in active chiral matter and elucidate its essential role in the anomalous Hall-like effect.

| S-EPMC9586265 | biostudies-literature

Viscosity Landscape of Phase-Separated Lipid Membrane Estimated from Fluid Velocity Field.

Project description:In cell membranes, the functional constituents such as peptides, proteins, and polysaccharides diffuse in a sea of lipids as single molecules and molecular aggregates. Thus, the fluidity of the heterogeneous multicomponent membrane is important for understanding the roles of the membrane in cell functionality. Recently, Henle and Levine described the hydrodynamics of molecular diffusion in a spherical membrane. A tangential point force at the north pole induces a pair of vortices whose centers lie on a line perpendicular to the point force and are symmetrical with respect to the point force. The position of the vortex center depends on ηm/Rηw, where R is the radius of the spherical membrane, and ηm and ηw are the viscosities of the membrane and the surrounding medium, respectively. Based on this theoretical prediction, we applied a point force to a phase-separated spherical vesicle composed of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine/1,2-dioleoyl-sn-glycero-3-phosphocholine/cholesterol by means of a microinjection technique. The pathlines were visualized by trajectories of microdomains. We determined the position of the vortex center and estimated the membrane viscosity using the dependence of the position of the vortex center on ηm/Rηw. The obtained apparent membrane viscosities for various compositions are mapped on the phase diagram. The membrane viscosity is almost constant in the range of 0 <ϕLo ≤ 0.5 (ϕLo: area fraction of the liquid ordered phase), whereas that in the range of 0.5 ≤ ϕLo < 1.0 exponentially increases with increase of ϕLo. The obtained viscosity landscape provides a basic understanding of the fluidity of heterogeneous multicomponent membranes.

| S-EPMC7136286 | biostudies-literature

Low-viscosity matrix suspension culture enables scalable analysis of patient-derived organoids and tumoroids from the large intestine.

Project description:Cell embedment into a solid support matrix is considered essential for the culture of intestinal epithelial organoids and tumoroids, but this technique presents challenges that impede scalable culture expansion, experimental manipulation, high-throughput screening and diagnostic applications. We have developed a low-viscosity matrix (LVM) suspension culture method that enables efficient establishment and propagation of organoids and tumoroids from the human large intestine. Organoids and tumoroids cultured in LVM suspension recapitulate the morphological development observed in solid matrices, with tumoroids reflecting the histological features and genetic heterogeneity of primary colorectal cancers. We demonstrate the utility of LVM suspension culture for organoid and tumoroid bioreactor applications and biobanking, as well as tumoroid high-throughput drug sensitivity testing. These methods provide opportunities for the study and use of patient-derived organoids and tumoroids from the large intestine.

| S-EPMC8438070 | biostudies-literature

Viscosity measurement dataset for a water-based drilling mud-carbon nanotube suspension at high-pressure and high-temperature.

Project description:This data article presents the measured viscosity of a carbon nanotube (CNT) suspension in water-based drilling mud, also termed as nano-muds ("Rheology of a colloidal suspension of carbon nanotube particles in a water-based drilling fluid" Anoop et al., 2019). The apparent viscosity values of the nano-mud samples are measured using a high-pressure high-temperature viscometer at different shear rates, working based on a rotor and bob technique. The pressure and temperature of the samples are independently varied during the measurements from ambient conditions to 171 MPa and 176 °C, respectively, within two experimental schedules. Viscosity measurements for varying nanoparticle concentration, shear rate, pressure, and temperature are reported here for different CNT concentrations.

| S-EPMC6463761 | biostudies-literature

Measuring the Soret coefficient of nanoparticles in a dilute suspension.

Project description:Thermophoresis is an efficient process for the manipulation of molecules and nanoparticles due to the strong force it generates on the nanoscale. Thermophoresis is characterized by the Soret coefficient. Conventionally, the Soret coefficient of nanosized species is obtained by fitting the concentration profile under a temperature gradient at the steady state to a continuous phase model. However, when the number density of the target is ultralow and the dispersed species cannot be treated as a continuous phase, the bulk concentration fluctuates spatially, preventing extraction of temperature-gradient induced concentration profile. The present work demonstrates a strategy to tackle this problem by superimposing snapshots of nanoparticle distribution. The resulting image is suitable for the extraction of the Soret coefficient through the conventional data fitting method. The strategy is first tested through a discrete phase model that illustrates the spatial fluctuation of the nanoparticle concentration in a dilute suspension in response to the temperature gradient. By superimposing snapshots of the stochastic distribution, a thermophoretic depletion profile with low standard error is constructed, indicative of the Soret coefficient. Next, confocal analysis of nanoparticle distribution in response to a temperature gradient is performed using polystyrene nanobeads down to 1e-5% (v/v). The experimental results also reveal that superimposing enhances the accuracy of extracted Soret coefficient. The critical particle number density in the superimposed image for predicting the Soret coefficient is hypothesized to depend on the spatial resolution of the image. This study also demonstrates that the discrete phase model is an effective tool to study particle migration under thermophoresis in the liquid phase.

| S-EPMC4160128 | biostudies-literature

Frequency-dependent viscosity of salmon ovarian fluid has biophysical implications for sperm-egg interactions.

Project description:Gamete-level sexual selection of externally fertilising species is usually achieved by modifying sperm behaviour with mechanisms that alter the chemical environment in which gametes perform. In fish, this can be accomplished through the ovarian fluid, a substance released with the eggs at spawning. While the biochemical effects of ovarian fluid in relation to sperm energetics have been investigated, the influence of the physical environment in which sperm compete remains poorly explored. Our objective was therefore to gain insights on the physical structure of this fluid and potential impacts on reproduction. Using soft-matter physics approaches of steady-state and oscillatory viscosity measurements, we subjected wild Atlantic salmon ovarian fluids to variable shear stresses and frequencies resembling those exerted by sperm swimming through the fluid near eggs. We show that this fluid, which in its relaxed state is a gel-like substance, displays a non-Newtonian viscoelastic and shear-thinning profile, where the viscosity decreases with increasing shear rates. We concurrently find that this fluid obeys the Cox-Merz rule below 7.6 Hz and infringes it above this level, thus indicating a shear-thickening phase where viscosity increases provided it is probed gently enough. This suggests the presence of a unique frequency-dependent structural network with relevant implications for sperm energetics and fertilisation dynamics. This article has an associated ECR Spotlight interview with Marco Graziano.

| S-EPMC10086386 | biostudies-literature

Surface engineering of nanoparticles in suspension for particle based bio-sensing.

Project description:Surface activation of nanoparticles in suspension using amino organosilane has been carried out via strict control of a particle surface ad-layer of water using a simple but efficient protocol 'Tri-phasic Reverse Emulsion' (TPRE). This approach produced thin and ordered layers of particle surface functional groups which allowed the efficient conjugation of biomolecules. When used in bio-sensing applications, the resultant conjugates were highly efficient in the hybrid capture of complementary oligonucleotides and the detection of food borne microorganism. TPRE overcomes a number of fundamental problems associated with the surface modification of particles in aqueous suspension viz. particle aggregation, density and organization of resultant surface functional groups by controlling surface condensation of the aminosilane. The approach has potential for application in areas as diverse as nanomedicine, to food technology and industrial catalysis.

| S-EPMC3413008 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data