Potential use of additivity of mutational effects in simplifying protein engineering.
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ABSTRACT:
SUBMITTER: Skinner MM
PROVIDER: S-EPMC38227 | biostudies-other | 1996 Oct
REPOSITORIES: biostudies-other
Publications
Potential use of additivity of mutational effects in simplifying protein engineering.
Skinner M M MM Terwilliger T C TC
Proceedings of the National Academy of Sciences of the United States of America 19961001 20
The problem of rationally engineering protein molecules can be simplified where effects of mutations on protein function are additive. Crystal structures of single and double mutants in the hydrophobic core of gene V protein indicate that structural and functional effects of core mutations are additive when the regions structurally influenced by the mutations do not substantially overlap. These regions of influence can provide a simple basis for identifying sets of mutations that will show addit ...[more]
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