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Quantitative laser speckle flowmetry of the in vivo microcirculation using sidestream dark field microscopy.


ABSTRACT: We present integrated Laser Speckle Contrast Imaging (LSCI) and Sidestream Dark Field (SDF) flowmetry to provide real-time, non-invasive and quantitative measurements of speckle decorrelation times related to microcirculatory flow. Using a multi exposure acquisition scheme, precise speckle decorrelation times were obtained. Applying SDF-LSCI in vitro and in vivo allows direct comparison between speckle contrast decorrelation and flow velocities, while imaging the phantom and microcirculation architecture. This resulted in a novel analysis approach that distinguishes decorrelation due to flow from other additive decorrelation sources.

SUBMITTER: Nadort A 

PROVIDER: S-EPMC3829532 | biostudies-other | 2013

REPOSITORIES: biostudies-other

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