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Optical sectioning by wide-field photobleaching imprinting microscopy.


ABSTRACT: We present a generic wide-field optical sectioning scheme, photobleaching imprinting microscopy (PIM), for depth-resolved cross-sectional fluorescence imaging. Wide-field PIM works by extracting a nonlinear component that depends on the excitation fluence as a result of photobleaching-induced fluorescence decay. Since no specific fluorescent dyes or illumination modules are required, wide-field PIM is easy to implement on a standard microscope. Moreover, wide-field PIM is superior to deconvolution microscopy in removing background fluorescence, yielding a six-fold improvement in image contrast.

SUBMITTER: Li C 

PROVIDER: S-EPMC3829865 | biostudies-other | 2013 Oct

REPOSITORIES: biostudies-other

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