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Whole-cell screening-based identification of inhibitors against the intraphagosomal survival of Mycobacterium tuberculosis.

ABSTRACT: We describe here an efficient strategy that employs whole-cell-based screening and further short listing of the compounds by cytotoxicity- and fluorescence-based intracellular assays, resulting in potential bactericidal hits against the growth of Mycobacterium tuberculosis in broth culture as well as in phagosomes. These compounds also inhibited multidrug-resistant strains of M. tuberculosis but showed no or poor inhibition of nonpathogenic mycobacteria or other bacterial species such as Escherichia coli.

SUBMITTER: Khare G 

PROVIDER: S-EPMC3837871 | biostudies-other | 2013 Dec

REPOSITORIES: biostudies-other

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Whole-cell screening-based identification of inhibitors against the intraphagosomal survival of Mycobacterium tuberculosis.

Khare Garima G   Kumar Praveen P   Tyagi Anil K AK  

Antimicrobial agents and chemotherapy 20130923 12

We describe here an efficient strategy that employs whole-cell-based screening and further short listing of the compounds by cytotoxicity- and fluorescence-based intracellular assays, resulting in potential bactericidal hits against the growth of Mycobacterium tuberculosis in broth culture as well as in phagosomes. These compounds also inhibited multidrug-resistant strains of M. tuberculosis but showed no or poor inhibition of nonpathogenic mycobacteria or other bacterial species such as Escheri  ...[more]