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High-density distributed electrode network, a multi-functional electroporation method for delivery of molecules of different sizes.

ABSTRACT: We present a multi-functional electroporation method for delivery of biomolecule utilizing a high-density distributed electrode network (HDEN) under tri-phase electric stimulation. The HDEN device, with which drastic pH change during the electroporation was avoided,was demonstrated to be highly effective for transfection of not only DNA plasmids and small interfering RNAs (siRNA), but also a small molecular anti-cancer drug, into cells in adjustable volumes of cell suspension. The method constitutes a very flexible electroporation approach in a wide range of in vitro or ex vivo scenarios in various tubes, standard multi-well plates as well as flow chambers.

SUBMITTER: Wu M

PROVIDER: S-EPMC3842547 | biostudies-other | 2013

REPOSITORIES: biostudies-other

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High-density distributed electrode network, a multi-functional electroporation method for delivery of molecules of different sizes.

Wu Mengxi M Zhao Deyao D Zhong Wenfeng W Yan Hao H Wang Xiaoxia X Liang Zicai Z Li Zhihong Z

Scientific reports 20131128

We present a multi-functional electroporation method for delivery of biomolecule utilizing a high-density distributed electrode network (HDEN) under tri-phase electric stimulation. The HDEN device, with which drastic pH change during the electroporation was avoided,was demonstrated to be highly effective for transfection of not only DNA plasmids and small interfering RNAs (siRNA), but also a small molecular anti-cancer drug, into cells in adjustable volumes of cell suspension. The method constit ...[more]

PMID: 24284649

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High-density distributed electrode network, a multi-functional electroporation method for delivery of molecules of different sizes.

Publications

High-density distributed electrode network, a multi-functional electroporation method for delivery of molecules of different sizes.

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