Rapid immunoglobulin M-based dengue diagnostic test using surface plasmon resonance biosensor.
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ABSTRACT: Surface plasmon resonance (SPR) is a medical diagnosis technique with high sensitivity and specificity. In this research, a new method based on SPR is proposed for rapid, 10-minute detection of the anti-dengue virus in human serum samples. This novel technique, known as rapid immunoglobulin M (IgM)-based dengue diagnostic test, can be utilized quickly and easily at the point of care. Four dengue virus serotypes were used as ligands on a biochip. According to the results, a serum volume of only 1 μl from a dengue patient (as a minimized volume) is required to indicate SPR angle variation to determine the ratio of each dengue serotype in samples with 83-93% sensitivity and 100% specificity.
SUBMITTER: Jahanshahi P
PROVIDER: S-EPMC3900921 | biostudies-other | 2014
REPOSITORIES: biostudies-other
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