Ontology highlight
ABSTRACT:
SUBMITTER: Ciobanasu C
PROVIDER: S-EPMC3916842 | biostudies-other | 2014
REPOSITORIES: biostudies-other
Ciobanasu Corina C Faivre Bruno B Le Clainche Christophe C
Nature communications 20140101
The force generated by the actomyosin cytoskeleton controls focal adhesion dynamics during cell migration. This process is thought to involve the mechanical unfolding of talin to expose cryptic vinculin-binding sites. However, the ability of the actomyosin cytoskeleton to directly control the formation of a talin-vinculin complex and the resulting activity of the complex are not known. Here we develop a microscopy assay with pure proteins in which the self-assembly of actomyosin cables controls ...[more]