Ontology highlight
ABSTRACT:
SUBMITTER: Diatchenko L
PROVIDER: S-EPMC39182 | biostudies-other | 1996 Jun
REPOSITORIES: biostudies-other
Diatchenko L L Lau Y F YF Campbell A P AP Chenchik A A Moqadam F F Huang B B Lukyanov S S Lukyanov K K Gurskaya N N Sverdlov E D ED Siebert P D PD
Proceedings of the National Academy of Sciences of the United States of America 19960601 12
A new and highly effective method, termed suppression subtractive hybridization (SSH), has been developed for the generation of subtracted cDNA libraries. It is based primarily on a recently described technique called suppression PCR and combines normalization and subtraction in a single procedure. The normalization step equalizes the abundance of cDNAs within the target population and the subtraction step excludes the common sequences between the target and driver populations. In a model system ...[more]