Ontology highlight
ABSTRACT:
SUBMITTER: Dukowic-Schulze S
PROVIDER: S-EPMC3933774 | biostudies-other | 2014
REPOSITORIES: biostudies-other
Dukowic-Schulze Stefanie S Sundararajan Anitha A Ramaraj Thiruvarangan T Mudge Joann J Chen Changbin C
Frontiers in plant science 20140225
High-throughput sequencing has become the large-scale approach of choice to study global gene expression and the distribution of specific chromatin marks and features. However, the limited availability of large amounts of purified cells made it very challenging to apply sequencing-based techniques in plant meiosis research in the past. In this paper, we describe a method to isolate meiocytes from maize anthers and detailed protocols to successfully perform RNA-seq, smRNA-seq, H3K4me3-ChIP-seq, a ...[more]