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Triggered sorting and co-assembly of genetically engineered protein microdomains in the cytoplasm.

ABSTRACT: Elastin-like polypeptides (ELPs) are genetically encoded protein polymers that reversibly phase separate in response to stimuli. They respond sharply to small shifts in temperature and form dense microdomains in the living eukaryotic cytosol. For the first time, this communication illustrates how to tune the ELP sequence and architecture for either co-assembly or sorting of distinct proteins into microdomains within a living cell.

SUBMITTER: Shi P

PROVIDER: S-EPMC3947098 | biostudies-other | 2014 Jan

REPOSITORIES: biostudies-other

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Triggered sorting and co-assembly of genetically engineered protein microdomains in the cytoplasm.

Shi Pu P Lin Yi-An YA Pastuszka Martha M Cui Honggang H Mackay J Andrew JA

Advanced materials (Deerfield Beach, Fla.) 20131018 3

Elastin-like polypeptides (ELPs) are genetically encoded protein polymers that reversibly phase separate in response to stimuli. They respond sharply to small shifts in temperature and form dense microdomains in the living eukaryotic cytosol. For the first time, this communication illustrates how to tune the ELP sequence and architecture for either co-assembly or sorting of distinct proteins into microdomains within a living cell. ...[more]

PMID: 24136711

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