Human TOP3: a single-copy gene encoding DNA topoisomerase III.
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ABSTRACT:
SUBMITTER: Hanai R
PROVIDER: S-EPMC39666 | biostudies-other | 1996 Apr
REPOSITORIES: biostudies-other
Publications
Human TOP3: a single-copy gene encoding DNA topoisomerase III.
Hanai R R Caron P R PR Wang J C JC
Proceedings of the National Academy of Sciences of the United States of America 19960401 8
A human cDNA encoding a protein homologous to the Escherichia coli DNA topoisomerase I subfamily of enzymes has been identified through cloning and sequencing. Expressing the cloned human cDNA in yeast (delta)top1 cells lacking endogenous DNA topoisomerase I yielded an activity in cell extracts that specifically reduces the number of supercoils in a highly negatively supercoiled DNA. On the basis of these results, the human gene containing the cDNA sequence has been denoted TOP3, and the protein ...[more]
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