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Optimization of medium using response surface methodology for lipid production by Scenedesmus sp.

ABSTRACT: Lipid production is an important indicator for assessing microalgal species for biodiesel production. In this work, the effects of medium composition on lipid production by Scenedesmus sp. were investigated using the response surface methodology. The results of a Plackett-Burman design experiment revealed that NaHCO?, NaH?PO4·2H?O and NaNO? were three factors significantly influencing lipid production, which were further optimized by a Box-Behnken design. The optimal medium was found to contain 3.07 g L?¹ NaHCO?, 15.49 mg L?¹ NaH?PO4·2H?O and 803.21 mg L?¹ NaNO?. Using the optimal conditions previously determined, the lipid production (304.02 mg·L?¹) increased 54.64% more than that using the initial medium, which agreed well with the predicted value 309.50 mg L?¹. Additionally, lipid analysis found that palmitic acid (C16:0) and oleic acid (C18:1) dominantly constituted the algal fatty acids (about 60% of the total fatty acids) and a much higher content of neutral lipid accounted for 82.32% of total lipids, which strongly proved that Scenedesmus sp. is a very promising feedstock for biodiesel production.

SUBMITTER: Yang F

PROVIDER: S-EPMC3967207 | biostudies-other | 2014 Mar

REPOSITORIES: biostudies-other

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Optimization of medium using response surface methodology for lipid production by Scenedesmus sp.

Yang Fangfang F Long Lijuan L Sun Xiumei X Wu Hualian H Li Tao T Xiang Wenzhou W

Marine drugs 20140306 3

Lipid production is an important indicator for assessing microalgal species for biodiesel production. In this work, the effects of medium composition on lipid production by Scenedesmus sp. were investigated using the response surface methodology. The results of a Plackett-Burman design experiment revealed that NaHCO₃, NaH₂PO4·2H₂O and NaNO₃ were three factors significantly influencing lipid production, which were further optimized by a Box-Behnken design. The optimal medium was found to contain ...[more]

PMID: 24663113

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Project description:IntroductionA halophilic bacterium of the Halomonas elongata BK-AG25 has successfully produced ectoine with high productivity. To overcome the drawbacks of high levels of salt in the production process, a nonhalophilic bacteria of Escherichia coli (E. coli) was used to express the ectoine gene cluster of the halophilic bacteria, and the production of ectoine by the recombinant cell was optimized.MethodsThe ectoine gene cluster from the halophilic bacterium was isolated and inserted into an expression plasmid of pET30(a) and subsequently transformed into E. coli BL21 (DE3). Production of ectoine from the recombinant E. coli was investigated and then maximized by optimizing the level of nutrients in the medium, as well as the bioprocess conditions using response surface methodology. The experimental designs were performed using a central composite design.ResultsThe recombinant E. coli successfully expressed the ectoine gene cluster of Halomonas elongata BK-AG25 under the control of the T7 promoter. The recombinant cell was able to produce ectoine, of which most were excreted into the medium. The optimization of ectoine production with the response surface methodology showed that the level of salt in the medium, the incubation temperature, the optical density of the bacteria before induction, and the final concentration of the inducer gave a significant effect on ectoine production by the recombinant E. coli. Interestingly, the level of salt in the medium and the incubation temperature showed an inverse effect on the production of intracellular and extracellular ectoine by the recombinant cell. At the optimum conditions, the production yield was about 418 mg ectoine/g cdw (cell dry weight) after 12 hours of incubation.ConclusionThis study is the first report on the expression of an ectoine gene cluster of Halomonas elongata BK-AG25 in E. coli BL21, under the control of the T7 promoter. Optimization of the level of nutrients in the medium, as well as the bioprocess condition using response surface methodology, has successfully increased the production of ectoine by the recombinant bacteria.

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Optimization of medium using response surface methodology for lipid production by Scenedesmus sp.

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Optimization of medium using response surface methodology for lipid production by Scenedesmus sp.

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