Instantaneous three-dimensional sensing using spatial light modulator illumination with extended depth of field imaging.
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ABSTRACT: Imaging three-dimensional structures represents a major challenge for conventional microscopies. Here we describe a Spatial Light Modulator (SLM) microscope that can simultaneously address and image multiple targets in three dimensions. A wavefront coding element and computational image processing enables extended depth-of-field imaging. High-resolution, multi-site three-dimensional targeting and sensing is demonstrated in both transparent and scattering media over a depth range of 300-1,000 microns.
SUBMITTER: Quirin S
PROVIDER: S-EPMC3971059 | biostudies-other | 2013 Jul
REPOSITORIES: biostudies-other
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